Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559
نویسندگان
چکیده
منابع مشابه
Chlorophyll and cytochrome 6-559 content of the photochemical reaction center of photosystem II
Photosystem II reaction centers comprised of the Dl, D2 and cytochrome b-559 polypeptides were isolated from welldefined oxygen-evolving reaction center preparations using either a combination of LiClO, and dodecyhnaltoside, or Triton X-100 alone. Yields of chlorophyll and cytochrome b-559 for both preparative methods were compared, and pigment contents were compared based on 2.0 b-559 per reac...
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چکیده ندارد.
Isolation of photosystem II reaction center complexes from plants.
Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure...
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A new contact site between the two reaction center polypeptides D 1 and D 2 o f photosystem II close to arg 238 and arg234 respectively is proposed. The amino acid sequences involved are between the 4 th transmembrane and a connecting parallel helix. The sequence includes a tryp sin sensitive site in both polypeptides, the likely cleavage site in the rapid turnover o f the D 1 polypeptide and ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1987
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.84.1.109